Search results for " SIA"

2018

The bioactive coating of calcium phosphate cement (CPC) is a promising approach to enhance the bone-healing properties of bone substitutes. The purpose of this study was to evaluate whether coating CPCs with bone sialoprotein (BSP) results in increased bone formation. Forty-five female C57BL/6NRj mice with an average age of six weeks were divided into three groups. Either a BSP-coated or an uncoated three-dimensional plotted scaffold was implanted into a drilled 2.7-mm diameter calvarial defect, or the defect was left empty (control group; no CPC). Histological analyses revealed that BSP-coated scaffolds were better integrated into the local bone stock eight weeks after implantation. Bone v…

Effect of bone sialoprotein coated three-dimensional printed calcium phosphate scaffolds on primary human osteoblasts

2018

The combination of the two techniques of rapid prototyping 3D-plotting and bioactive surface functionalization is presented, with emphasis on the in vitro effect of Bone Sialoprotein (BSP) on primary human osteoblasts (hOBs). Our primary objective was to demonstrate the BSP influence on the expression of distinctive osteoblast markers in hOBs. Secondary objectives included examinations of the scaffolds' surface and the stability of BSP-coating as well as investigations of cell viability and proliferation. 3D-plotted calcium phosphate cement (CPC) scaffolds were coated with BSP via physisorption. hOBs were seeded on the coated scaffolds, followed by cell viability measurements, gene expressi…

2016

Orthopedic implant failure due to aseptic loosening and mechanical instability remains a major problem in total joint replacement. Improving osseointegration at the bone-implant interface may reduce micromotion and loosening. Bone sialoprotein (BSP) has been shown to enhance bone formation when coated onto titanium femoral implants and in rat calvarial defect models. However, the most appropriate method of BSP coating, the necessary level of BSP coating, and the effect of BSP coating on cell behavior remain largely unknown. In this study, BSP was covalently coupled to titanium surfaces via an aminosilane linker (APTES), and its properties were compared to BSP applied to titanium via physiso…

Interventional treatment of sialoliths in main salivary glands

2002

Purpose. The aim of our study was to demonstrate the effectiveness of interventional radiology in the treatment of sialolithiasis, as the first-choice treatment for the removal of stones located in the middle and proximal tracts of the main salivary ducts, and to assess its limitations and contraindications. Material and methods. Between February 1998 and May 2001 eleven interventional removals of sialoliths were performed for recurrent obstruction of the main salivary duct associated with chronic sialadenitis. Patients were selected on the basis of a preliminary sialogram, designed to determine the location and size of the stone. Exclusion criteria were location of the stone in the gland h…

ZS "Avotiņi" saimnieciskās darbības analīze un nākotnes attīstības perspektīvas

2016

Maģistra darbā ir veikta ZS “Avotiņi” saimnieciskā darba analīze, izmantojot sekojošas analīzes metodes: •pārskata lasīšana; •horizontālā analīze; •vertikālā analīze; •finanšu koeficientu aprēķināšana. Finanšu pārskatu lietotāji, veicot finanšu analīzi, var iegūt informāciju par uzņēmuma saimniecisko vai finanšu stāvokli. Tas ļauj pieņemt lēmumus par līdzekļu investēšanu, tādejādi jebkurš lēmums var būtiski ietekmēt tālāku saimniecisko un finansiālo darbību. Darba teorētiskajā daļā autore maģistra darbā apraksta finansiālā stāvokļa mērķus un metodes, raksturo informācijas avotus un lietotājus. Lai novērtētu uzņēmuma saistību racionālu izmantošanu, darba praktiskajā daļā tiek novērtēta bilan…

Silica as a morphogenetically active inorganic polymer.

2013

At present the scaffolds used for bioprinting of cells do not elicit morphogenetic responses in the cells. In the present study we approached a solution by studying the effect of an inorganic silica supplement added to an Na-alginate matrix. Bone- and osteoblast-like SaOS-2 cells were embedded into this organic polymeric matrix which was additionally enriched with 400 μM prehydrolyzed TEOS [tetra-ethoxy-silane], a source of ortho-silicate. In this silica-based matrix the cells synthesized hydroxyapatite crystallites after exposure to a mineralization activation cocktail composed of β-glycerophosphate, ascorbic acid and dexamethasone. The degree of hydroxyapatite synthesis, determined by sta…

Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold.

2021

(1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. N…

Covid-19, documento SIAARTI e gerarchie di cura: cambiamenti e comportamenti sanitari in Italia

2021

In periodi segnati da pandemie sanitarie e crisi economiche profonde, l’incertezza rende ansiosi, influenzando comportamenti singoli, collettivi. Il Covid-19 ha già provocato e comporterà una serie di mutamenti che in Italia toccano diversi aspetti e tra questi sicuramente si annovera il principio della cura, per cui l’uso delle risorse e le politiche sanitarie necessitano di un processo di analisi e approfondimento ad oggi non maturo. L’articolo pone sotto i riflettori un tema lasciato nell’ombra: il diritto alla cura e il principio di gerarchia che è stato applicato durante la pandemia (1). Pietra miliare è il documento della Società Italiana di Anestesia, Analgesia, Rianimazione e Terapi…

Selective sweep of Wolbachia and parthenogenetic host genomes – the example of the weevil Eusomus ovulum

2016

Most parthenogenetic weevil species are postulated to have originated via hybridization, but Wolbachia has also been speculated to play a role via the induction of parthenogenesis. Here, we examine the molecular diversity of Wolbachia and parthenogenetic host genomes. The host species studied here, Eusomus ovulum, is known to be exclusively parthenogenetic and triploid. The E. ovulum populations that we examined had a low genetic diversity of mitochondrial (cytochrome oxidase I gene) and nuclear markers (internal transcribed spacer 2 and elongation factor 1-α gene), and they all were infected by only single bacteria strains (genotyped for five genes according to the multilocus sequence typi…

Recensione a G. Alfano, A. Cortellessa, D. Dalmas, M. Di Gesù, S. Jossa, D. Scarpa: Dove siamo? Nuove posizioni della critica :duepunti Edizioni, Pal…

2011

G. Alfano, A. Cortellessa, D. Dalmas, M. Di Gesù, S. Jossa, D. Scarpa: Dove siamo? Nuove posizioni della critica :duepunti Edizioni, Palermo 2011. Collana Posizioni (0.1); pagine 128; prezzo € 15,00